Journal: iScience

Article Title: Prognostic value of BTG1 for predicting decitabine sensitivity in de novo acute myeloid leukemia

doi: 10.1016/j.isci.2025.114327

Figure Lengend Snippet: Downstream signaling pathway of BTG1 in AML cell lines (A) Top three signaling pathways in terms of Gene Ratio were EBV infection (0.037), Wnt signaling pathway (0.035), and hematopoietic lineage (0.023) of KEGG pathway enrichment. (B) Effect of BTG1 on the mRNA expression of β-catenin, Cyclin D1, and C-Myc. (C) Effect of BTG1 on the protein expression of β-catenin and Cyclin D1. (D) The relative viability of AML cells after interference of BTG1 and treatment with FH535. (E) Apoptosis of AML cells after interference of BTG1 and treatment with FH535. Data are presented as mean ± standard deviation (SD). (ns, p > 0.05; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001).

Article Snippet: Mouse monoclonal anti-human Cyclin D1 , Proteintech , Cat# 60186-1-Ig RRID: AB_10793718.

Techniques: Protein-Protein interactions, Infection, Expressing, Standard Deviation

Journal: Molecular and cellular endocrinology

Article Title: DUAL INHIBITION OF ERK1/2 AND AKT PATHWAYS IS REQUIRED TO SUPPRESS THE GROWTH AND SURVIVAL OF ENDOMETRIOTIC CELLS AND LESIONS

doi: 10.1016/j.mce.2018.12.011

Figure Lengend Snippet: Details of the antibody used.

Article Snippet: Anti-human mouse monoclonal Cyclin D1 , Cell Signaling , 2926 , 1:1000.

Techniques: Concentration Assay

Journal: PLoS ONE

Article Title: Neoadjuvant eribulin mesylate following anthracycline and taxane in triple negative breast cancer: Results from the HOPE study

doi: 10.1371/journal.pone.0220644

Figure Lengend Snippet: Specific reaction with β-catenin, N-cadherin, Cylin D1, c-myc and Zeb-1 monoclonal antibodies in primary TNBC before and after neoadjuvant therapy with AT followed by E. Intense immunostaining was evident in pre-treatment primary tumor biopsy, whereas it appeared reduced in surgical specimen after study treatment, with the unique exception of N-cadherin, whose levels remained stable. Scale bar , reported at the right bottom of each picture.

Article Snippet: After neutralization of the endogenous peroxidase with 3% H 2 O 2 and Fc blocking by a specific protein block (Novocastra UK) the samples were incubated overnight with the primary antibodies Rabbit Monoclonal anti-human β-catenin, dilution 1:100 pH8 [Clone 6B3; Cell Signaling]; Rabbit Polyclonal anti-human N-cadherin, dilution 1:500 pH9 [AbCam Code ab18203]; Rabbit Monoclonal anti-human c-myc, dilution 1:500 pH6 [Clone Y69; AbCam Code ab32072]; Mouse Monoclonal anti-human Zeb-1, dilution 1:150 pH6 [Clone 3G6; AbCam Code ab180905]; Mouse Monoclonal anti-human Cyclin D1, dilution 1:25 pH9 [Clone P2D11F11; Leica Novocastra], at 4 C°.

Techniques: Bioprocessing, Immunostaining